As an important part of the comprehensive treatment methods, the urate-lowering Chinese herbs may provide favorable clinical effects on hyperuricemia in its potential to invigorate spleen and take away dampness. Owing to the long-term duration, it delivered up the potential adverse reactions (ADRs) and issues about the drug-induced liver harm from these herbs. To tackle this problem, the bioinformatics methods which combined the network pharmacology, computer simulation and molecular biology experiments were undertaken to elucidate the underlying drug-induced liver injury molecular mechanisms of uratelowering Chinese herbs. Several digital databases have been searched to pick out the plausible liver injury compounds in published research. Then, the putative target profile of liver injury was predicted, and the interaction network was constructed based on the links between the compounds, corresponding targets and core pathways.